Id: | acc3899 |
Group: | 1sens |
Protein: | Chk2 |
Gene Symbol: | CHEK2 |
Protein Id: | O96017 |
Protein Name: | CHK2_HUMAN |
PTM: | phosphorylation |
Site: | Thr68 |
Site Sequence: | TLSSLETVSTQELYSIPEDQE |
Disease Category: | Cancer |
Disease: | Colorectal Cancer |
Disease Subtype: | |
Disease Cellline: | HT-29 |
Disease Info: | |
Drug: | fisetin + radiation |
Drug Info: | Fisetin is a natural flavonoid with potential antioxidant and anti - inflammatory properties | Radiation is a physical therapy method often used in cancer treatment to kill cancer cells. |
Effect: | suppress |
Effect Info: | Fisetin can enhance the radiosensitivity of human colon cancer HT–29 cells with p53 mutation by downregulating protein phosphorylation. |
Note: | drug comb |
Score: | 4.0 |
Pubmed(PMID): | 20637980 |
Sentence Index: | 20637980_4 |
Sentence: | "Western blot analysis was performed to ascertain the protein levels of gamma-H2AX, phospho-Chk2, active caspase-3, PARP cleavage, phospho-p38, phospho-AKT, and phospho-ERK1/2. RESULTS: Fisetin pretreatment enhanced the radiosensitivity of p53-mutant HT-29 human colorectal cancer cells but not human keratocyte HaCaT cells; it also prolonged radiation-induced G(2)/M arrest, enhanced radiation-induced cell growth arrest in HT-29 cells, and suppressed radiation-induced phospho-H2AX (Ser-139) and phospho-Chk2 (Thr-68) in p53-mutant HT-29 cells. Fisetin could potentially be developed as a novel radiosensitizer against radioresistant human cancer cells." |
Sequence & Structure:
MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL
Select PDB:
Target | Drug name | MOA | Phase | Status | Disease | Source |
---|---|---|---|---|---|---|
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 2 | Completed | small cell lung carcinoma | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 2 | Completed | cancer | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 2 | Terminated | breast cancer | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 2 | Terminated | ovarian cancer | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 2 | Completed | ovarian cancer | ClinicalTrials |
CHEK2 | XL-844 | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Terminated | chronic lymphocytic leukemia | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | myelodysplastic syndrome | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | acute myeloid leukemia | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Active, not recruiting | central nervous system cancer | ClinicalTrials |
CHEK2 | XL-844 | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Terminated | lymphoma | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | neoplasm | ClinicalTrials ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | squamous cell lung carcinoma | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | squamous cell carcinoma | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Active, not recruiting | medulloblastoma | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | non-small cell lung carcinoma | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | colorectal neoplasm | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | upper aerodigestive tract neoplasm | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | metastasis | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | Central Nervous System Neoplasm | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | chronic myelomonocytic leukemia | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Active, not recruiting | brain cancer | ClinicalTrials |
CHEK2 | PREXASERTIB | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Completed | cancer | ClinicalTrials ClinicalTrials ClinicalTrials |
CHEK2 | XL-844 | Serine/threonine-protein kinase Chk2 inhibitor | 1 | Terminated | cancer | ClinicalTrials |
Note: Only show clinically investigational or approved drugs with protein targets.
Protein Tractability:
source: Open TargetsPTM Intensity:
source: CPTACNo intensity data of this site,
show all other sites!
CHEK2-Ser163 | |||||
---|---|---|---|---|---|
Cancer | Intensity | ||||
BRCA | |||||
COAD | |||||
HGSC | |||||
ccRCC | |||||
GBM | |||||
HNSC | 0.165 | ||||
LUAD | 0.907 | ||||
LUSC | -1.072 | ||||
non_ccRCC | |||||
PDAC | |||||
UCEC |
CHEK2-Thr430 | |||||
---|---|---|---|---|---|
Cancer | Intensity | ||||
BRCA | |||||
COAD | |||||
HGSC | -0.707 | ||||
ccRCC | |||||
GBM | |||||
HNSC | |||||
LUAD | 0.707 | ||||
LUSC | |||||
non_ccRCC | |||||
PDAC | |||||
UCEC |
CHEK2-Thr432 | |
---|---|
Cancer | Intensity |
BRCA | |
COAD | |
HGSC | -1.151 |
ccRCC | |
GBM | |
HNSC | 0.655 |
LUAD | 0.496 |
LUSC | |
non_ccRCC | |
PDAC | |
UCEC |
PTM-Disease Association:
source: PTMDResidue | Position | State | Disease | Class | PMID |
---|---|---|---|---|---|
T | 68 | D | Colorectal cancer | Phosphorylation | 33500399 |
T | 68 | U | Colorectal carcinoma | Phosphorylation | 35999268 |
T | 68 | U | Gastric cancer | Phosphorylation | 33747205 |
T | 68 | U | Bladder cancer | Phosphorylation | 15361851 |
T | 68 | U | Bloom syndrome | Phosphorylation | 17634426 |
T | 68 | U | Non-small cell lung cancer/carcinoma | Phosphorylation | 16705183 |
State Note: Based on the distinct PTM states in diseases, PTMD classified all disease-associated PTMs into six classes, including whether the up-regulation (U) or down-regulation (D) of PTM levels, the absence (A) or presence (P) of PTMs, and the creation (C) or disruption (N) of PTM sites are associated with diseases.
PTM-Drug Perturbation Response:
source: DecryptMNo data.
Function score:
source: funscoRNo data.